Total IgE and eotaxin (CCL11) contents in tears of patients suffering from seasonal allergic conjunctivitis.

TitreTotal IgE and eotaxin (CCL11) contents in tears of patients suffering from seasonal allergic conjunctivitis.
Publication TypeJournal Article
Year of Publication2014
AuthorsEperon, S, Berguiga, M, Ballabeni, P, Guex-Crosier, C, Guex-Crosier, Y
JournalGraefe's Archive For Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie
Volume252
Issue9
Pagination1359-1367
Date Published09/2014
DOI10.1007/s00417-014-2683-6
ISSN0721-832X (linking)
ISBN Number1435-702X (Electronic)
Mots-clésAdolescent, Adult, Aged, Chemokine CCL11, Conjunctivitis, Allergic, Enzyme-Linked Immunosorbent Assay, Eye Proteins, Female, Humans, Immunoglobulin E, Male, Middle Aged, Pollen, Prospective Studies, Seasons, Tears, Young Adult
Abstract

BACKGROUND: To prospectively investigate patients with seasonal allergic conjunctivitis (SAC) during the pollen season and test associations between tears total IgE, eotaxin concentrations, and SAC severity.

METHODS: Enrolled patients presented ocular symptoms and clinical signs of SAC at the time of presentation. Ocular itching, hyperaemia, chemosis, eyelid swelling, and tearing were scored, and the sum of these scores was defined as the clinical score. Conjunctival papillae were separately graded. We measured eotaxin concentration in tears by an enzyme-linked immunosorbent assay (ELISA) and total tear IgE by Lacrytest strip.

RESULTS: Among thirty patients (30 eyes), 11 showed neither tear IgE nor tear eotaxin, while 15 out of 19 patients with positive IgE values presented a positive amount of eotaxin in their tears (Fisher's test: p < 0.001). The mean eotaxin concentration was 641 ± 154 (SEM) pg/ml. In patients with no amount of tear IgE, we observed a lower conjunctival papilla grade than in patients whose tears contained some amount of IgE (trend test: p = 0.032). In the 15 patients whose tear eotaxin concentration was null, tear IgE concentration was 5.3 ± 3.5 arbitrary units; in the other 15 patients whose eotaxin was positive, IgE reached 21 ± 4.3 arbitrary U (Mann-Whitney: p < 0.001). We measured 127 ± 47 pg/ml eotaxin in patients with no history of SAC but newly diagnosed as suffering from SAC, and 852 ± 218 pg/ml eotaxin in patients with a known SAC (p = 0.008). In contrast, tear IgE concentrations of both groups did not differ statistically significantly (p = 0.947).

CONCLUSIONS: If IgE and eotaxin secreted in tears are major contributors in SAC pathogenesis, they however act at different steps of the process.

Notes

Publication types: JOURNAL ARTICLE

Alternate URL

http://www.ncbi.nlm.nih.gov/pubmed/24916929?dopt=Abstract

First publication date (online)

06/2014

WOS ID (UT)

000341709000002

Alternate JournalGraefes Arch. Clin. Exp. Ophthalmol.
Citation Key / SERVAL ID3578
Peer reviewRefereed
PubMed ID24916929
PubMed Central IDPMC4153979
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